Welcome

Experts in transepithelial electrophysiology: electronics, electrode design and Ussing chambers experiments.

EP Design focuses at the design of equipment for electrophysiological experiments with epithelia: cultured monolayers, native tissues from animals and biopsies. Initially we developed equipment for studies of these tissues mounted in Ussing chambers. More recently we focused our efforts on high throughput electrophysiological assays with monolayers cultured on Costar 24 and Millipore 96 transwells. Electrode arrays and multichannel clamp amplifiers have been designed to make simultaneous measurements of open circuit potential (PD), transepithelial resistance (TEER) and short circuit current (Isc) of 24 epithelia.

Experiences

Over the last 30 years, EP-Design has acquired experience with transepithelial electrophysiological measurements:

  • Transepithelial voltage and current clamp technology.
  • Data acquisition of short-circuit current, transepithelial conductance and voltage.
  • High precision conductance measurements with low frequency sine waves.
  • Recording of transepithelial impedance with frequencies up to 64KHz.
  • Recording of solution resistance with high frequency impedance (leaky epithelia with R<50 Ohm).
  • Noise analysis.